Construct: shRNA TRCN0000319543

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007945.2-2522s21c1
DNA Barcode:
GTAATGGAAGCTCCGAGTTAC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Eps8 (13860)
Gene Description:
epidermal growth factor receptor pathway substrate 8
Transcript:
RefSeq NM_007945.2 (NON-CURRENT)
Match location:
Position 2522 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000319543)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTAATGGAAGCTCCGAGTTAC
Hairpin Sequence:
5'-CCGG-GTAATGGAAGCTCCGAGTTAC-CTCGAG-GTAACTCGGAGCTTCCATTAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTAATGGAAGCTCCGAGTTACCTCGAGGTAACTCGGAGCTTCCATTACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTAATGGAAGCTCCGAGTTACCTCGAGGTAACTCGGAGCTTCCATTAC-3'

Other clones with same target sequence:

(none)