Construct: shRNA TRCN0000319690

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_027182.2-654s21c1
DNA Barcode:
CGTACTCTTCTCAGACAAGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Trip13 (69716)
Gene Description:
thyroid hormone receptor interactor 13
Transcript:
RefSeq NM_027182.2 (CURRENT)
Match location:
Position 654 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000319690)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTACTCTTCTCAGACAAGAA
Hairpin Sequence:
5'-CCGG-CGTACTCTTCTCAGACAAGAA-CTCGAG-TTCTTGTCTGAGAAGAGTACG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTACTCTTCTCAGACAAGAACTCGAGTTCTTGTCTGAGAAGAGTACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTACTCTTCTCAGACAAGAACTCGAGTTCTTGTCTGAGAAGAGTACG-3'

Other clones with same target sequence:

  1. TRCN0000024990