Construct: shRNA TRCN0000319699

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011049.4-981s21c1
DNA Barcode:
GCCAACATCGTCACACTACAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cdk16 (18555)
Gene Description:
cyclin-dependent kinase 16
Transcript:
RefSeq NM_011049.4 (NON-CURRENT)
Match location:
Position 981 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000319699)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCAACATCGTCACACTACAT
Hairpin Sequence:
5'-CCGG-GCCAACATCGTCACACTACAT-CTCGAG-ATGTAGTGTGACGATGTTGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCAACATCGTCACACTACATCTCGAGATGTAGTGTGACGATGTTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCAACATCGTCACACTACATCTCGAGATGTAGTGTGACGATGTTGGC-3'

Other clones with same target sequence:

  1. TRCN0000023078