Construct: shRNA TRCN0000319774

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_027470.3-1354s21c1
DNA Barcode:
CGCAAGCAGCAAAGACGTGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pak4 (70584)
Gene Description:
p21 protein (Cdc42/Rac)-activated kinase 4
Transcript:
RefSeq NM_027470.3 (CURRENT)
Match location:
Position 1354 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000319774)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCAAGCAGCAAAGACGTGAA
Hairpin Sequence:
5'-CCGG-CGCAAGCAGCAAAGACGTGAA-CTCGAG-TTCACGTCTTTGCTGCTTGCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCAAGCAGCAAAGACGTGAACTCGAGTTCACGTCTTTGCTGCTTGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCAAGCAGCAAAGACGTGAACTCGAGTTCACGTCTTTGCTGCTTGCG-3'

Other clones with same target sequence:

  1. TRCN0000025233