Construct: shRNA TRCN0000319834

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178398.4-526s21c1
DNA Barcode:
GTCGCTCTACATACACAACAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Wipi2 (74781)
Gene Description:
WD repeat domain, phosphoinositide interacting 2
Transcript:
RefSeq NM_178398.4 (CURRENT)
Match location:
Position 526 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000319834)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTCGCTCTACATACACAACAT
Hairpin Sequence:
5'-CCGG-GTCGCTCTACATACACAACAT-CTCGAG-ATGTTGTGTATGTAGAGCGAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTCGCTCTACATACACAACATCTCGAGATGTTGTGTATGTAGAGCGACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTCGCTCTACATACACAACATCTCGAGATGTTGTGTATGTAGAGCGAC-3'

Other clones with same target sequence:

  1. TRCN0000178373