Construct: shRNA TRCN0000320009

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_181070.5-217s21c1
DNA Barcode:
GATGGAAATAAGGCTAAACTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rab18 (19330)
Gene Description:
RAB18, member RAS oncogene family
Transcript:
RefSeq NM_181070.5 (NON-CURRENT)
Match location:
Position 217 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000320009)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATGGAAATAAGGCTAAACTT
Hairpin Sequence:
5'-CCGG-GATGGAAATAAGGCTAAACTT-CTCGAG-AAGTTTAGCCTTATTTCCATC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATGGAAATAAGGCTAAACTTCTCGAGAAGTTTAGCCTTATTTCCATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATGGAAATAAGGCTAAACTTCTCGAGAAGTTTAGCCTTATTTCCATC-3'

Other clones with same target sequence:

  1. TRCN0000177028