Construct: shRNA TRCN0000320103

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_175036.4-389s21c1
DNA Barcode:
GAAGAGGAGATGATTTCAGTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Leprot (230514)
Gene Description:
leptin receptor overlapping transcript
Transcript:
RefSeq NM_175036.4 (CURRENT)
Match location:
Position 389 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000320103)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAGAGGAGATGATTTCAGTT
Hairpin Sequence:
5'-CCGG-GAAGAGGAGATGATTTCAGTT-CTCGAG-AACTGAAATCATCTCCTCTTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAGAGGAGATGATTTCAGTTCTCGAGAACTGAAATCATCTCCTCTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAGAGGAGATGATTTCAGTTCTCGAGAACTGAAATCATCTCCTCTTC-3'

Other clones with same target sequence:

  1. TRCN0000174934