Construct: shRNA TRCN0000320169

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_027478.3-406s21c1
DNA Barcode:
CCTTTCTCATAATCATAGGCT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tmem230 (70612)
Gene Description:
transmembrane protein 230
Transcript:
RefSeq NM_027478.3 (CURRENT)
Match location:
Position 406 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000320169)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTTTCTCATAATCATAGGCT
Hairpin Sequence:
5'-CCGG-CCTTTCTCATAATCATAGGCT-CTCGAG-AGCCTATGATTATGAGAAAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTTTCTCATAATCATAGGCTCTCGAGAGCCTATGATTATGAGAAAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTTTCTCATAATCATAGGCTCTCGAGAGCCTATGATTATGAGAAAGG-3'

Other clones with same target sequence:

  1. TRCN0000192250