Construct: shRNA TRCN0000320177

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145589.2-1935s21c1
DNA Barcode:
GAGCGCAATGGAACTCTGATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Prr14 (233895)
Gene Description:
proline rich 14
Transcript:
RefSeq NM_145589.2 (CURRENT)
Match location:
Position 1935 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000320177)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGCGCAATGGAACTCTGATT
Hairpin Sequence:
5'-CCGG-GAGCGCAATGGAACTCTGATT-CTCGAG-AATCAGAGTTCCATTGCGCTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGCGCAATGGAACTCTGATTCTCGAGAATCAGAGTTCCATTGCGCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGCGCAATGGAACTCTGATTCTCGAGAATCAGAGTTCCATTGCGCTC-3'

Other clones with same target sequence:

  1. TRCN0000190549