Construct: shRNA TRCN0000320193

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010309.3-3229s21c1
DNA Barcode:
GCCAAGTACTTCATTCGGGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gnas (14683)
Gene Description:
GNAS (guanine nucleotide binding protein, alpha stimulating) complex locus
Transcript:
RefSeq NM_010309.3 (NON-CURRENT)
Match location:
Position 3229 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000320193)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCAAGTACTTCATTCGGGAT
Hairpin Sequence:
5'-CCGG-GCCAAGTACTTCATTCGGGAT-CTCGAG-ATCCCGAATGAAGTACTTGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCAAGTACTTCATTCGGGATCTCGAGATCCCGAATGAAGTACTTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCAAGTACTTCATTCGGGATCTCGAGATCCCGAATGAAGTACTTGGC-3'

Other clones with same target sequence:

  1. TRCN0000115059