Construct: shRNA TRCN0000320211

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011865.3-1105s21c1
DNA Barcode:
CCATGAACTCACCATTCCAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pcbp1 (23983)
Gene Description:
poly(rC) binding protein 1
Transcript:
RefSeq NM_011865.3 (NON-CURRENT)
Match location:
Position 1105 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000320211)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCATGAACTCACCATTCCAAA
Hairpin Sequence:
5'-CCGG-CCATGAACTCACCATTCCAAA-CTCGAG-TTTGGAATGGTGAGTTCATGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCATGAACTCACCATTCCAAACTCGAGTTTGGAATGGTGAGTTCATGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCATGAACTCACCATTCCAAACTCGAGTTTGGAATGGTGAGTTCATGG-3'

Other clones with same target sequence:

  1. TRCN0000120940