Construct: shRNA TRCN0000320315

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_028439.1-357s21c1
DNA Barcode:
GCATTCTTCTGTGGAGAAGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
3110009E18Rik (73103)
Gene Description:
RIKEN cDNA 3110009E18 gene
Transcript:
RefSeq NM_028439.1 (NON-CURRENT)
Match location:
Position 357 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000320315)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCATTCTTCTGTGGAGAAGAT
Hairpin Sequence:
5'-CCGG-GCATTCTTCTGTGGAGAAGAT-CTCGAG-ATCTTCTCCACAGAAGAATGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCATTCTTCTGTGGAGAAGATCTCGAGATCTTCTCCACAGAAGAATGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCATTCTTCTGTGGAGAAGATCTCGAGATCTTCTCCACAGAAGAATGC-3'

Other clones with same target sequence:

  1. TRCN0000181881