Construct: shRNA TRCN0000320410

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003909.3-1754s21c1
DNA Barcode:
CCACGAAACAACAGAAGCAGT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CPNE3 (8895)
Gene Description:
copine 3
Transcript:
RefSeq NM_003909.3 (NON-CURRENT)
Match location:
Position 1754 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000320410)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACGAAACAACAGAAGCAGT
Hairpin Sequence:
5'-CCGG-CCACGAAACAACAGAAGCAGT-CTCGAG-ACTGCTTCTGTTGTTTCGTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACGAAACAACAGAAGCAGTCTCGAGACTGCTTCTGTTGTTTCGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACGAAACAACAGAAGCAGTCTCGAGACTGCTTCTGTTGTTTCGTGG-3'

Other clones with same target sequence:

  1. TRCN0000000718