Construct: shRNA TRCN0000320458

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006377.3-1957s21c1
DNA Barcode:
GAAGATCCGAGAGCGAAATAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
UNC13B (10497)
Gene Description:
unc-13 homolog B
Transcript:
RefSeq NM_006377.3 (NON-CURRENT)
Match location:
Position 1957 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000320458)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAGATCCGAGAGCGAAATAA
Hairpin Sequence:
5'-CCGG-GAAGATCCGAGAGCGAAATAA-CTCGAG-TTATTTCGCTCTCGGATCTTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAGATCCGAGAGCGAAATAACTCGAGTTATTTCGCTCTCGGATCTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAGATCCGAGAGCGAAATAACTCGAGTTATTTCGCTCTCGGATCTTC-3'

Other clones with same target sequence:

(none)