Construct: shRNA TRCN0000320524

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024539.3-1282s21c1
DNA Barcode:
GCAGTGGATGTTATTCCTCAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RNF128 (79589)
Gene Description:
ring finger protein 128
Transcript:
RefSeq NM_024539.3 (CURRENT)
Match location:
Position 1282 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000320524)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGTGGATGTTATTCCTCAT
Hairpin Sequence:
5'-CCGG-GCAGTGGATGTTATTCCTCAT-CTCGAG-ATGAGGAATAACATCCACTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGTGGATGTTATTCCTCATCTCGAGATGAGGAATAACATCCACTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGTGGATGTTATTCCTCATCTCGAGATGAGGAATAACATCCACTGC-3'

Other clones with same target sequence:

  1. TRCN0000004797