Construct: shRNA TRCN0000320555

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002843.3-1527s21c1
DNA Barcode:
ACGAGTCGTCATCTAACTATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PTPRJ (5795)
Gene Description:
protein tyrosine phosphatase receptor type J
Transcript:
RefSeq NM_002843.3 (NON-CURRENT)
Match location:
Position 1527 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000320555)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACGAGTCGTCATCTAACTATA
Hairpin Sequence:
5'-CCGG-ACGAGTCGTCATCTAACTATA-CTCGAG-TATAGTTAGATGACGACTCGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACGAGTCGTCATCTAACTATACTCGAGTATAGTTAGATGACGACTCGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACGAGTCGTCATCTAACTATACTCGAGTATAGTTAGATGACGACTCGT-3'

Other clones with same target sequence:

(none)