Construct: shRNA TRCN0000320558

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_022073.3-690s21c1
DNA Barcode:
GTGGCTTGCTATCCGGGAAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
EGLN3 (112399)
Gene Description:
egl-9 family hypoxia inducible factor 3
Transcript:
RefSeq NM_022073.3 (NON-CURRENT)
Match location:
Position 690 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000320558)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTGGCTTGCTATCCGGGAAAT
Hairpin Sequence:
5'-CCGG-GTGGCTTGCTATCCGGGAAAT-CTCGAG-ATTTCCCGGATAGCAAGCCAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTGGCTTGCTATCCGGGAAATCTCGAGATTTCCCGGATAGCAAGCCACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTGGCTTGCTATCCGGGAAATCTCGAGATTTCCCGGATAGCAAGCCAC-3'

Other clones with same target sequence:

  1. TRCN0000001050