Construct: shRNA TRCN0000320623

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019887.4-1087s21c1
DNA Barcode:
TGCGTTGATTGAAGCTATTAC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DIABLO (56616)
Gene Description:
diablo IAP-binding mitochondrial protein
Transcript:
RefSeq NM_019887.4 (NON-CURRENT)
Match location:
Position 1087 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000320623)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGCGTTGATTGAAGCTATTAC
Hairpin Sequence:
5'-CCGG-TGCGTTGATTGAAGCTATTAC-CTCGAG-GTAATAGCTTCAATCAACGCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGCGTTGATTGAAGCTATTACCTCGAGGTAATAGCTTCAATCAACGCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGCGTTGATTGAAGCTATTACCTCGAGGTAATAGCTTCAATCAACGCA-3'

Other clones with same target sequence:

(none)