Construct: shRNA TRCN0000320696

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002758.3-1455s21c1
DNA Barcode:
GGCCTTGGAATCTATAGTATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MAP2K6 (5608)
Gene Description:
mitogen-activated protein kinase kinase 6
Transcript:
RefSeq NM_002758.3 (NON-CURRENT)
Match location:
Position 1455 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000320696)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGCCTTGGAATCTATAGTATA
Hairpin Sequence:
5'-CCGG-GGCCTTGGAATCTATAGTATA-CTCGAG-TATACTATAGATTCCAAGGCC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGCCTTGGAATCTATAGTATACTCGAGTATACTATAGATTCCAAGGCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGCCTTGGAATCTATAGTATACTCGAGTATACTATAGATTCCAAGGCC-3'

Other clones with same target sequence:

(none)