Construct: shRNA TRCN0000320745

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016542.3-2182s21c1
DNA Barcode:
CCTCTTGGTGTATAGTATTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
STK26 (51765)
Gene Description:
serine/threonine kinase 26
Transcript:
RefSeq NM_016542.3 (NON-CURRENT)
Match location:
Position 2182 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000320745)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCTTGGTGTATAGTATTTA
Hairpin Sequence:
5'-CCGG-CCTCTTGGTGTATAGTATTTA-CTCGAG-TAAATACTATACACCAAGAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCTTGGTGTATAGTATTTACTCGAGTAAATACTATACACCAAGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCTTGGTGTATAGTATTTACTCGAGTAAATACTATACACCAAGAGG-3'

Other clones with same target sequence:

  1. TRCN0000003191