Construct: shRNA TRCN0000320831

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005160.3-2470s21c1
DNA Barcode:
CTCCTACTTATCACGTAAATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GRK3 (157)
Gene Description:
G protein-coupled receptor kinase 3
Transcript:
RefSeq NM_005160.3 (NON-CURRENT)
Match location:
Position 2470 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000320831)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTCCTACTTATCACGTAAATT
Hairpin Sequence:
5'-CCGG-CTCCTACTTATCACGTAAATT-CTCGAG-AATTTACGTGATAAGTAGGAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTCCTACTTATCACGTAAATTCTCGAGAATTTACGTGATAAGTAGGAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTCCTACTTATCACGTAAATTCTCGAGAATTTACGTGATAAGTAGGAG-3'

Other clones with same target sequence:

(none)