Construct: shRNA TRCN0000320911

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024083.2-1812s21c1
DNA Barcode:
CCAGCAGGAATAAAGACTTGT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ASPSCR1 (79058)
Gene Description:
ASPSCR1 tether for SLC2A4, UBX domain containing
Transcript:
RefSeq NM_024083.2 (NON-CURRENT)
Match location:
Position 1812 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000320911)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGCAGGAATAAAGACTTGT
Hairpin Sequence:
5'-CCGG-CCAGCAGGAATAAAGACTTGT-CTCGAG-ACAAGTCTTTATTCCTGCTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGCAGGAATAAAGACTTGTCTCGAGACAAGTCTTTATTCCTGCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGCAGGAATAAAGACTTGTCTCGAGACAAGTCTTTATTCCTGCTGG-3'

Other clones with same target sequence:

  1. TRCN0000022414