Construct: shRNA TRCN0000320926

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_177559.2-738s21c1
DNA Barcode:
CGTAAACAACACAGACTTCAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CSNK2A1 (1457)
Gene Description:
casein kinase 2 alpha 1
Transcript:
RefSeq NM_177559.2 (NON-CURRENT)
Match location:
Position 738 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000320926)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTAAACAACACAGACTTCAA
Hairpin Sequence:
5'-CCGG-CGTAAACAACACAGACTTCAA-CTCGAG-TTGAAGTCTGTGTTGTTTACG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTAAACAACACAGACTTCAACTCGAGTTGAAGTCTGTGTTGTTTACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTAAACAACACAGACTTCAACTCGAGTTGAAGTCTGTGTTGTTTACG-3'

Other clones with same target sequence:

  1. TRCN0000000607
  2. TRCN0000010673