Construct: shRNA TRCN0000321035

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007772.2-1738s21c1
DNA Barcode:
CGGTATCGAATCCCGAATCTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Hivep1 (110521)
Gene Description:
human immunodeficiency virus type I enhancer binding protein 1
Transcript:
RefSeq NM_007772.2 (CURRENT)
Match location:
Position 1738 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000321035)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGTATCGAATCCCGAATCTT
Hairpin Sequence:
5'-CCGG-CGGTATCGAATCCCGAATCTT-CTCGAG-AAGATTCGGGATTCGATACCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGTATCGAATCCCGAATCTTCTCGAGAAGATTCGGGATTCGATACCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGTATCGAATCCCGAATCTTCTCGAGAAGATTCGGGATTCGATACCG-3'

Other clones with same target sequence:

  1. TRCN0000009800