Construct: shRNA TRCN0000321058

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008862.3-1471s21c1
DNA Barcode:
GCCCTTTCATACCCAAATGTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pkia (18767)
Gene Description:
protein kinase inhibitor, alpha
Transcript:
RefSeq NM_008862.3 (CURRENT)
Match location:
Position 1471 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000321058)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCCTTTCATACCCAAATGTT
Hairpin Sequence:
5'-CCGG-GCCCTTTCATACCCAAATGTT-CTCGAG-AACATTTGGGTATGAAAGGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCCTTTCATACCCAAATGTTCTCGAGAACATTTGGGTATGAAAGGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCCTTTCATACCCAAATGTTCTCGAGAACATTTGGGTATGAAAGGGC-3'

Other clones with same target sequence:

  1. TRCN0000012463