Construct: shRNA TRCN0000321210

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011547.3-591s21c1
DNA Barcode:
ACGTAGAAGACCCGGGTATTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tfap2a (21418)
Gene Description:
transcription factor AP-2, alpha
Transcript:
RefSeq NM_011547.3 (NON-CURRENT)
Match location:
Position 591 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000321210)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACGTAGAAGACCCGGGTATTA
Hairpin Sequence:
5'-CCGG-ACGTAGAAGACCCGGGTATTA-CTCGAG-TAATACCCGGGTCTTCTACGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACGTAGAAGACCCGGGTATTACTCGAGTAATACCCGGGTCTTCTACGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACGTAGAAGACCCGGGTATTACTCGAGTAATACCCGGGTCTTCTACGT-3'

Other clones with same target sequence:

(none)