Construct: shRNA TRCN0000321236
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_008332.3-2151s21c1
- DNA Barcode:
- ATAGTTTCCACAGTAGTAAAT
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Ifit2 (15958)
- Gene Description:
- interferon-induced protein with tetratricopeptide repeats 2
- Transcript:
- RefSeq NM_008332.3 (CURRENT)
- Match location:
- Position 2151 (3UTR)
Vector Information:
- Vector Backbone:
- pLKO_005
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000321236)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- ATAGTTTCCACAGTAGTAAAT
- Hairpin Sequence:
-
5'-CCGG-ATAGTTTCCACAGTAGTAAAT-CTCGAG-ATTTACTACTGTGGAAACTAT-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGATAGTTTCCACAGTAGTAAATCTCGAGATTTACTACTGTGGAAACTATTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAATAGTTTCCACAGTAGTAAATCTCGAGATTTACTACTGTGGAAACTAT-3'
Other clones with same target sequence:
(none)
