Construct: shRNA TRCN0000321335

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008302.3-1307s21c1
DNA Barcode:
CCGCAAGAACATCGTCAAGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Hsp90ab1 (15516)
Gene Description:
heat shock protein 90 alpha (cytosolic), class B member 1
Transcript:
RefSeq NM_008302.3 (CURRENT)
Match location:
Position 1307 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000321335)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGCAAGAACATCGTCAAGAA
Hairpin Sequence:
5'-CCGG-CCGCAAGAACATCGTCAAGAA-CTCGAG-TTCTTGACGATGTTCTTGCGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGCAAGAACATCGTCAAGAACTCGAGTTCTTGACGATGTTCTTGCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGCAAGAACATCGTCAAGAACTCGAGTTCTTGACGATGTTCTTGCGG-3'

Other clones with same target sequence:

  1. TRCN0000011442