Construct: shRNA TRCN0000321358

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007496.2-12096s21c1
DNA Barcode:
ACCTATCTATGCCGGTGATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Zfhx3 (11906)
Gene Description:
zinc finger homeobox 3
Transcript:
RefSeq NM_007496.2 (CURRENT)
Match location:
Position 12096 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000321358)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACCTATCTATGCCGGTGATTT
Hairpin Sequence:
5'-CCGG-ACCTATCTATGCCGGTGATTT-CTCGAG-AAATCACCGGCATAGATAGGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACCTATCTATGCCGGTGATTTCTCGAGAAATCACCGGCATAGATAGGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACCTATCTATGCCGGTGATTTCTCGAGAAATCACCGGCATAGATAGGT-3'

Other clones with same target sequence:

(none)