Construct: shRNA TRCN0000321561

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_153049.2-2537s21c1
DNA Barcode:
AGCACATGGATGATCTGTTTG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mkl1 (223701)
Gene Description:
MKL (megakaryoblastic leukemia)/myocardin-like 1
Transcript:
RefSeq NM_153049.2 (NON-CURRENT)
Match location:
Position 2537 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000321561)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGCACATGGATGATCTGTTTG
Hairpin Sequence:
5'-CCGG-AGCACATGGATGATCTGTTTG-CTCGAG-CAAACAGATCATCCATGTGCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGCACATGGATGATCTGTTTGCTCGAGCAAACAGATCATCCATGTGCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGCACATGGATGATCTGTTTGCTCGAGCAAACAGATCATCCATGTGCT-3'

Other clones with same target sequence:

(none)