Construct: shRNA TRCN0000321735

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_013880.3-2404s21c1
DNA Barcode:
CCCTTATGTCTATGTGGAAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Plcl2 (224860)
Gene Description:
phospholipase C-like 2
Transcript:
RefSeq NM_013880.3 (CURRENT)
Match location:
Position 2404 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000321735)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTTATGTCTATGTGGAAAT
Hairpin Sequence:
5'-CCGG-CCCTTATGTCTATGTGGAAAT-CTCGAG-ATTTCCACATAGACATAAGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTTATGTCTATGTGGAAATCTCGAGATTTCCACATAGACATAAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTTATGTCTATGTGGAAATCTCGAGATTTCCACATAGACATAAGGG-3'

Other clones with same target sequence:

  1. TRCN0000026929