Construct: shRNA TRCN0000321753

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009594.3-354s21c1
DNA Barcode:
CTCCGGGTCTTGGGTTATAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Abl1 (11350)
Gene Description:
c-abl oncogene 1, non-receptor tyrosine kinase
Transcript:
RefSeq NM_009594.3 (NON-CURRENT)
Match location:
Position 354 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000321753)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTCCGGGTCTTGGGTTATAAT
Hairpin Sequence:
5'-CCGG-CTCCGGGTCTTGGGTTATAAT-CTCGAG-ATTATAACCCAAGACCCGGAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTCCGGGTCTTGGGTTATAATCTCGAGATTATAACCCAAGACCCGGAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTCCGGGTCTTGGGTTATAATCTCGAGATTATAACCCAAGACCCGGAG-3'

Other clones with same target sequence:

(none)