Construct: shRNA TRCN0000321793

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009974.3-1186s21c1
DNA Barcode:
TGATATCCTGGGACAACATTC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Csnk2a2 (13000)
Gene Description:
casein kinase 2, alpha prime polypeptide
Transcript:
RefSeq NM_009974.3 (CURRENT)
Match location:
Position 1186 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000321793)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGATATCCTGGGACAACATTC
Hairpin Sequence:
5'-CCGG-TGATATCCTGGGACAACATTC-CTCGAG-GAATGTTGTCCCAGGATATCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGATATCCTGGGACAACATTCCTCGAGGAATGTTGTCCCAGGATATCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGATATCCTGGGACAACATTCCTCGAGGAATGTTGTCCCAGGATATCA-3'

Other clones with same target sequence:

(none)