Construct: shRNA TRCN0000321800

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007864.3-1128s21c1
DNA Barcode:
GATCAGTCATAGCAGCTACTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Dlg4 (13385)
Gene Description:
discs, large homolog 4 (Drosophila)
Transcript:
RefSeq NM_007864.3 (CURRENT)
Match location:
Position 1128 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000321800)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATCAGTCATAGCAGCTACTT
Hairpin Sequence:
5'-CCGG-GATCAGTCATAGCAGCTACTT-CTCGAG-AAGTAGCTGCTATGACTGATC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATCAGTCATAGCAGCTACTTCTCGAGAAGTAGCTGCTATGACTGATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATCAGTCATAGCAGCTACTTCTCGAGAAGTAGCTGCTATGACTGATC-3'

Other clones with same target sequence:

  1. TRCN0000221740