Construct: shRNA TRCN0000321872

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010121.2-3474s21c1
DNA Barcode:
CCATGAGTTCATCTGGAACAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Eif2ak3 (13666)
Gene Description:
eukaryotic translation initiation factor 2 alpha kinase 3
Transcript:
RefSeq NM_010121.2 (NON-CURRENT)
Match location:
Position 3474 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000321872)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCATGAGTTCATCTGGAACAA
Hairpin Sequence:
5'-CCGG-CCATGAGTTCATCTGGAACAA-CTCGAG-TTGTTCCAGATGAACTCATGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCATGAGTTCATCTGGAACAACTCGAGTTGTTCCAGATGAACTCATGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCATGAGTTCATCTGGAACAACTCGAGTTGTTCCAGATGAACTCATGG-3'

Other clones with same target sequence:

  1. TRCN0000028787