Construct: shRNA TRCN0000321885

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007936.3-3232s21c1
DNA Barcode:
TAAACCTGGAGCCAGTAATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Epha4 (13838)
Gene Description:
Eph receptor A4
Transcript:
RefSeq NM_007936.3 (CURRENT)
Match location:
Position 3232 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000321885)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TAAACCTGGAGCCAGTAATTT
Hairpin Sequence:
5'-CCGG-TAAACCTGGAGCCAGTAATTT-CTCGAG-AAATTACTGGCTCCAGGTTTA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTAAACCTGGAGCCAGTAATTTCTCGAGAAATTACTGGCTCCAGGTTTATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATAAACCTGGAGCCAGTAATTTCTCGAGAAATTACTGGCTCCAGGTTTA-3'

Other clones with same target sequence:

(none)