Construct: shRNA TRCN0000321889

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010141.3-3026s21c1
DNA Barcode:
AGAATGGTTGCAAGCTATTAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Epha7 (13841)
Gene Description:
Eph receptor A7
Transcript:
RefSeq NM_010141.3 (NON-CURRENT)
Match location:
Position 3026 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000321889)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGAATGGTTGCAAGCTATTAA
Hairpin Sequence:
5'-CCGG-AGAATGGTTGCAAGCTATTAA-CTCGAG-TTAATAGCTTGCAACCATTCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGAATGGTTGCAAGCTATTAACTCGAGTTAATAGCTTGCAACCATTCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGAATGGTTGCAAGCTATTAACTCGAGTTAATAGCTTGCAACCATTCT-3'

Other clones with same target sequence:

(none)