Construct: shRNA TRCN0000321925

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008778.2-329s21c1
DNA Barcode:
TACTCCAAACCTCCAACATAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pak3 (18481)
Gene Description:
p21 protein (Cdc42/Rac)-activated kinase 3
Transcript:
RefSeq NM_008778.2 (NON-CURRENT)
Match location:
Position 329 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000321925)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TACTCCAAACCTCCAACATAA
Hairpin Sequence:
5'-CCGG-TACTCCAAACCTCCAACATAA-CTCGAG-TTATGTTGGAGGTTTGGAGTA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTACTCCAAACCTCCAACATAACTCGAGTTATGTTGGAGGTTTGGAGTATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATACTCCAAACCTCCAACATAACTCGAGTTATGTTGGAGGTTTGGAGTA-3'

Other clones with same target sequence:

(none)