Construct: shRNA TRCN0000321937

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011074.2-1259s21c1
DNA Barcode:
GCCTTAGACAAGCATGGAATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cdk14 (18647)
Gene Description:
cyclin-dependent kinase 14
Transcript:
RefSeq NM_011074.2 (NON-CURRENT)
Match location:
Position 1259 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000321937)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTTAGACAAGCATGGAATA
Hairpin Sequence:
5'-CCGG-GCCTTAGACAAGCATGGAATA-CTCGAG-TATTCCATGCTTGTCTAAGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTTAGACAAGCATGGAATACTCGAGTATTCCATGCTTGTCTAAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTTAGACAAGCATGGAATACTCGAGTATTCCATGCTTGTCTAAGGC-3'

Other clones with same target sequence:

  1. TRCN0000023241