Construct: shRNA TRCN0000321978

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010513.2-4258s21c1
DNA Barcode:
CCAACGAGCAAGTTCTTCGTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Igf1r (16001)
Gene Description:
insulin-like growth factor I receptor
Transcript:
RefSeq NM_010513.2 (CURRENT)
Match location:
Position 4258 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000321978)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAACGAGCAAGTTCTTCGTT
Hairpin Sequence:
5'-CCGG-CCAACGAGCAAGTTCTTCGTT-CTCGAG-AACGAAGAACTTGCTCGTTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAACGAGCAAGTTCTTCGTTCTCGAGAACGAAGAACTTGCTCGTTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAACGAGCAAGTTCTTCGTTCTCGAGAACGAAGAACTTGCTCGTTGG-3'

Other clones with same target sequence:

  1. TRCN0000023493