Construct: shRNA TRCN0000322038

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001113464.1-745s21c1
DNA Barcode:
TTGGTGGAGAGCGAGAGATAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tec (21682)
Gene Description:
tec protein tyrosine kinase
Transcript:
RefSeq NM_001113464.1 (NON-CURRENT)
Match location:
Position 745 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000322038)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTGGTGGAGAGCGAGAGATAA
Hairpin Sequence:
5'-CCGG-TTGGTGGAGAGCGAGAGATAA-CTCGAG-TTATCTCTCGCTCTCCACCAA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTGGTGGAGAGCGAGAGATAACTCGAGTTATCTCTCGCTCTCCACCAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTGGTGGAGAGCGAGAGATAACTCGAGTTATCTCTCGCTCTCCACCAA-3'

Other clones with same target sequence:

(none)