Construct: shRNA TRCN0000322067

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011903.3-2535s21c1
DNA Barcode:
CGAAAGGAAGATCGCATTGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tlk2 (24086)
Gene Description:
tousled-like kinase 2 (Arabidopsis)
Transcript:
RefSeq NM_011903.3 (NON-CURRENT)
Match location:
Position 2535 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000322067)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGAAAGGAAGATCGCATTGAT
Hairpin Sequence:
5'-CCGG-CGAAAGGAAGATCGCATTGAT-CTCGAG-ATCAATGCGATCTTCCTTTCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGAAAGGAAGATCGCATTGATCTCGAGATCAATGCGATCTTCCTTTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGAAAGGAAGATCGCATTGATCTCGAGATCAATGCGATCTTCCTTTCG-3'

Other clones with same target sequence:

  1. TRCN0000026976