Construct: shRNA TRCN0000322228

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016788.3-4136s21c1
DNA Barcode:
TGTGGAGTGTTGGACAGAATG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tnk2 (51789)
Gene Description:
tyrosine kinase, non-receptor, 2
Transcript:
RefSeq NM_016788.3 (CURRENT)
Match location:
Position 4136 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000322228)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGTGGAGTGTTGGACAGAATG
Hairpin Sequence:
5'-CCGG-TGTGGAGTGTTGGACAGAATG-CTCGAG-CATTCTGTCCAACACTCCACA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGTGGAGTGTTGGACAGAATGCTCGAGCATTCTGTCCAACACTCCACATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGTGGAGTGTTGGACAGAATGCTCGAGCATTCTGTCCAACACTCCACA-3'

Other clones with same target sequence:

(none)