Construct: shRNA TRCN0000322242

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_153554.1-2695s21c1
DNA Barcode:
GCTAATGTTCACACTCCATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Aldh18a1 (56454)
Gene Description:
aldehyde dehydrogenase 18 family, member A1
Transcript:
RefSeq NM_153554.1 (NON-CURRENT)
Match location:
Position 2695 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000322242)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTAATGTTCACACTCCATTT
Hairpin Sequence:
5'-CCGG-GCTAATGTTCACACTCCATTT-CTCGAG-AAATGGAGTGTGAACATTAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTAATGTTCACACTCCATTTCTCGAGAAATGGAGTGTGAACATTAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTAATGTTCACACTCCATTTCTCGAGAAATGGAGTGTGAACATTAGC-3'

Other clones with same target sequence:

  1. TRCN0000025917