Construct: shRNA TRCN0000322250

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_023057.4-1248s21c1
DNA Barcode:
GAGAAGCTAACCCACGATTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Map3k20 (65964)
Gene Description:
mitogen-activated protein kinase kinase kinase 20
Transcript:
RefSeq NM_023057.4 (NON-CURRENT)
Match location:
Position 1248 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000322250)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGAAGCTAACCCACGATTAT
Hairpin Sequence:
5'-CCGG-GAGAAGCTAACCCACGATTAT-CTCGAG-ATAATCGTGGGTTAGCTTCTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGAAGCTAACCCACGATTATCTCGAGATAATCGTGGGTTAGCTTCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGAAGCTAACCCACGATTATCTCGAGATAATCGTGGGTTAGCTTCTC-3'

Other clones with same target sequence:

(none)