Construct: shRNA TRCN0000322267

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_028800.3-1484s21c1
DNA Barcode:
CACAACGCTATCTGCGGAAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Stk40 (74178)
Gene Description:
serine/threonine kinase 40
Transcript:
RefSeq NM_028800.3 (CURRENT)
Match location:
Position 1484 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000322267)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CACAACGCTATCTGCGGAAAT
Hairpin Sequence:
5'-CCGG-CACAACGCTATCTGCGGAAAT-CTCGAG-ATTTCCGCAGATAGCGTTGTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCACAACGCTATCTGCGGAAATCTCGAGATTTCCGCAGATAGCGTTGTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACACAACGCTATCTGCGGAAATCTCGAGATTTCCGCAGATAGCGTTGTG-3'

Other clones with same target sequence:

  1. TRCN0000220727