Construct: shRNA TRCN0000322368

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_153785.3-804s21c1
DNA Barcode:
CCTTCCTAGCAGTTCCGATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cdkl3 (213084)
Gene Description:
cyclin-dependent kinase-like 3
Transcript:
RefSeq NM_153785.3 (NON-CURRENT)
Match location:
Position 804 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000322368)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTTCCTAGCAGTTCCGATTT
Hairpin Sequence:
5'-CCGG-CCTTCCTAGCAGTTCCGATTT-CTCGAG-AAATCGGAACTGCTAGGAAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTTCCTAGCAGTTCCGATTTCTCGAGAAATCGGAACTGCTAGGAAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTTCCTAGCAGTTCCGATTTCTCGAGAAATCGGAACTGCTAGGAAGG-3'

Other clones with same target sequence:

  1. TRCN0000023138