Construct: shRNA TRCN0000322486
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_008960.2-1159s21c1
- DNA Barcode:
- GCTAGAACTTATCAAACCCTT
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Pten (19211)
- Gene Description:
- phosphatase and tensin homolog
- Transcript:
- RefSeq NM_008960.2 (CURRENT)
- Match location:
- Position 1159 (CDS)
Vector Information:
- Vector Backbone:
- pLKO_005
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000322486)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GCTAGAACTTATCAAACCCTT
- Hairpin Sequence:
-
5'-CCGG-GCTAGAACTTATCAAACCCTT-CTCGAG-AAGGGTTTGATAAGTTCTAGC-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGCTAGAACTTATCAAACCCTTCTCGAGAAGGGTTTGATAAGTTCTAGCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGCTAGAACTTATCAAACCCTTCTCGAGAAGGGTTTGATAAGTTCTAGC-3'
Other clones with same target sequence:
-
TRCN0000028992
