Construct: shRNA TRCN0000322500

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_130451.2-1424s21c1
DNA Barcode:
CTGGGCAGCTAACCTCTTTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Slc2a10 (170441)
Gene Description:
solute carrier family 2 (facilitated glucose transporter), member 10
Transcript:
RefSeq NM_130451.2 (NON-CURRENT)
Match location:
Position 1424 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000322500)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTGGGCAGCTAACCTCTTTAT
Hairpin Sequence:
5'-CCGG-CTGGGCAGCTAACCTCTTTAT-CTCGAG-ATAAAGAGGTTAGCTGCCCAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTGGGCAGCTAACCTCTTTATCTCGAGATAAAGAGGTTAGCTGCCCAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTGGGCAGCTAACCTCTTTATCTCGAGATAAAGAGGTTAGCTGCCCAG-3'

Other clones with same target sequence:

(none)