Construct: shRNA TRCN0000322533

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019700.4-1663s21c1
DNA Barcode:
GCTTCATAACCTCAGGATATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pus1 (56361)
Gene Description:
pseudouridine synthase 1
Transcript:
RefSeq NM_019700.4 (CURRENT)
Match location:
Position 1663 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000322533)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTTCATAACCTCAGGATATT
Hairpin Sequence:
5'-CCGG-GCTTCATAACCTCAGGATATT-CTCGAG-AATATCCTGAGGTTATGAAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTTCATAACCTCAGGATATTCTCGAGAATATCCTGAGGTTATGAAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTTCATAACCTCAGGATATTCTCGAGAATATCCTGAGGTTATGAAGC-3'

Other clones with same target sequence:

  1. TRCN0000120142